| DATE | TIME | |
| 16-DEC-2006 | 16:00 | start of the experiment
80.4g Artemia salt
20.0g Salt from the saline solar salt works in Cervia (Salina di Cervia)
in 1 liter of Sorgente Linda italian non-carbonated mineral water. |
| | 23:15 | added 2 small scoops of artemia parthenogenetica from cervia.
"Bubbler on" with a new much more fine grained structure, which produces much finer bubbles.
Airstone about 2 cm below the surface to avoid "shooting" the nauplii or adults to the surface and maybe hurt them |
| 17-DEC-2006 | 15:20 | added halogen lamp |
| | 18:00 | spotted first emerging nauplii (just hatched, but still in the membrane of the egg) |
| 18-DEC-2006 | - | In the oxygen experiment (with a salinity of 32g/l), first nauplii were visible after about
29 hours. Now they will need a little longer, since the salinity is 3 times as high. |
| 19-DEC-2006 | 15:00 | FIRST NAUPLIUS spotted!!! :-))) Seems to be just 2-3 hours old. |
| | 22:00 | About 10 nauplii visible |
| 20-DEC-2006 | 23:00 | Too many nauplii to count... at least 50 visible. |
| 21-DEC-2006 | 23:00 | added 2 drops of liquid algae - just to be sure that as soon as the nauplii have
consumed their yolk, they have "atomized" algae to eat |
| 23-DEC-2006 | 13:00 | added 3 drops of liquid algae, so that the nauplii have enough to feed during Xmas. |
| 26-DEC-2006 | 17:00 | Only about 5 metanauplii left, so I added another 3 small scoops of Artemia eggs from Cervia.
Since this experiment is not to show survival, but the salinity/color relationship, the
further addition of eggs does no harm to the result of the experiment. |
| 28-DEC-2006 | 22:00 |
No new nauplii visible - I switched on the halogen lamp again to engage hatching.
|
| 30-DEC-2006 | 23:00 | Plenty of nauplii are
visible |
| 04-JAN-2007 | 17:00 | A few nauplii survived.
Added one small scoop algae powder. |
| 05-JAN-2007 | 17:00 | Yesterdays small scoop algae powder
was too early for the nauplii, so I added 2 drops of liquid algae. |
| 06-JAN-2007 | 23:00 | Spotted about 10+ nauplii
and metanauplii from an age of nearly fresh hatched to about a week. |
| 08-JAN-2007 | 23:00 | Used a pipette to move 2
nauplii from the 32g/l microscopy tank to this experimental bottle. |
| 09-JAN-2007 | 22:00 | Counted 5 animals:
The almost adult one, one large metanauplius and 3 nauplii (among those 3 presumably the 2 I moved into
the bottle yesterday). |
| 10-JAN-2007 | 23:00 |
Added half a small scoop algae powder and one drop liquid algae powder. |
| 11-JAN-2007 | 23:00 | Used a pipette to move 2
nauplii from the 32g/l microscopy tank to this experimental bottle. |
| 17-JAN-2007 | 16:00 | One animal left (~7mm length) - it
doesn't show any reddish coloring yet |
| 25-JAN-2007 | 01:00 | One animal left - it
doesn't show any reddish coloring yet, but seems to feed well (excrement tail) |
| 26-JAN-2007 | 20:00 | There are 2 animals!!!
One is 7mm long, the other one 9mm!! Added 1 small scoop of algae powder. |
| 29-JAN-2007 | 23:00 | The 2 are still going
strong but do not show any signs of getting red. |
| 09-FEB-2007 | 16:00 | The 2 are still doing
fine, have "threads" on their tail (excrementaion, which indicates that they feed well), but do not
show any signs of getting red. On the contrary: the 2 are just pale. In tank #2 the Artemia show some
faint red coloration, since tank #2 is not aerated constantly and the oxygen level is lower than
in this experimental tank, which is (was) constantly aerated. Now I've turned off aeration to see
if they get red as in the "low-oxygen-tube" (which had a very low surface area, hence the water
had a extremely low oxygen level and the one and only adult Artemia got a bright red color). Also added
half a small scoop algae powder. |
| 01-MAR-2007 | 23:00 | The 2 are still going
strong without any aeration and feeding since 09-FEB-2007. One is still pale, but the other one
starts to show some faint redish coloration, so the oyxgen content is finally going down to levels
where increased haemoglobin production in Artemia starts. |
This is the experiment to determine if salinity is responsible for the
RED COLOR OF ARTEMIA. There are some "theories" that might explain the
red color of Artemia in some cases:
